LAUSR

Veh100200 0.0 0.5 1.0 1.5 Adult mice were injected once intraperitoneally with TX (25, 50, 100, or 200 mg/kg) or vehicle (Veh) and corpus tissue was analyzed 1 or 3 days later for parietal cells and cellular proliferation. TX-induced gastric injury was apparent in hematoxylin and eosin–stained sections, with parietal cell loss shown by H/K-ATPase-a immunostaining (SupplementaryFigure1 andFigure1A). Measurement of parietal cell Atp4a mRNA revealed a graded response (Figure 1B). The lowest dose, 25 mg/kg, showed no parietal cell loss, with extensive damage observed with 100–200 mg/kg, and intermediate loss at 50 mg/kg. The proliferative response,