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PURPOSE To establish the agreement of routine measurements taken before cataract surgery between the Aladdin biometer, which is based on optical low-coherence interferometry (OLCI), and the Lenstar biometer, which is based on optical low-coherence reflectometry (OLCR). SETTING Virginia Eye Consultants, Norfolk, Virginia, USA. DESIGN Prospective case series. METHODS Biometry parameters in the same eye were measured using the OLCI biometer and the OLCR biometer at separate preoperative visits. Measured parameters included axial length (AL), anterior chamber depth (ACD), keratometry (K) values (flat K, steep K, steep K axis), lens thickness, central corneal thickness (CCT), and the white-to-white (WTW) distance. Adverse events were monitored. RESULTS Data were collected for 101 eyes (65 right eyes) of 101 patients (mean age 67.9 years ± 7.2 [SD]). The mean values measured by the OLCI biometer and OLCR biometer, respectively, were as follows: AL, 23.88 ± 1.23 mm and 23.89 ± 1.23 mm; ACD, 3.24 ± 0.36 mm and 3.25 ± 0.37 mm; flat K, 43.63 ± 1.65 diopters (D) and 43.61 ± 1.67 D; steep K, 44.59 ± 1.76 D and 44.61 ± 1.77 D; lens thickness, 4.61 ± 0.39 mm and 4.45 ± 0.45 mm; CCT, 0.55 ± 0.04 mm and 0.55 ± 0.04 mm; and WTW distance, 11.63 ± 0.41 and 12.03 ± 0.49 mm. There was a high correlation between all parameter measurements obtained by the 2 devices. No adverse events occurred. CONCLUSION The measurements of the OLCI biometer were as reliable and reproducible as those of a leading biometer on the market, with the former having the advantage of an incorporated full corneal topographer.