LAUSR

Abstract Research has indicated that Moringa oleifera (MO) leaves aqueous extract possesses wound healing activity. However, the wound delivery of a plant aqueous extracts is often poor due to the large molecular sizes and poor lipid solubility of the polyphenols. In this study, a phyto-phospholipid complex (phytosome) containing MO aqueous extract was developed to maximize topical wound delivery of MO. Phytosomes containing MO (MOPCT) were formulated and characterised in terms of morphology, particle size, polydispersity, zeta potential, and encapsulation efficiency. In vitro normal human dermal fibroblast cells (NHDF) cytotoxicity and cell migration assay was also carried out on filtered MOPCT and controls. Under TEM, the MOPCT appeared as multilamellar vesicles with an average size of 198 ± 21 nm and a zeta potential −28.30 ± 1.31 mV. The encapsulation efficiency showed that quercetin (82.8%) kaempferol (52.2%), chlorogenic acid (15.6%), rosmarinic acid (8.44%) were present in the filtered MOPCT. The filtered MOPCT exhibited the highest NHDF cell migration and proliferation rate compared to the controls. The MOPCT also did not cause cytotoxicity at concentrations