LAUSR

Abstract Co-precipitated nanoaggregates (NAs) of small-molecule hydrophobic drugs have been widely used as carrier-free drug co-delivery systems, but how to construct stable NAs for potential co-delivery of combination drugs has been remaining unclear. Herein, we performed such investigation using our previously reported fluorescence resonance energy transfer (FRET) method, which was based on the fluorescent hydrophobic drugs of camptothecin (CPT), curcumin (CUR), and their lipophilic derivatives containing different alkyl chains. It is shown that unmodified CPT and CUR failed to form stable co-assembling NAs. Increasing hydrophobicity of one drug by conjugating an alkyl chain could promote the co-assembling ability of CPT and CUR, but the resulting NAs were very unstable and undergone a burst disassembly in the plasma. Only simultaneously enhancing the hydrophobicity of CPT and CUR could obtain stable co-assembling NAs. These results highlighted the co-assembly and disassembly of NAs formed by various hydrophobic drugs, which can effectively guide the design of co-assembling small-molecule drug co-delivery systems with high stability to resist the dilution of blood after intravenous injection.