LAUSR

Abstract Porous gold (Au) electrodes prepared by anodization of Au electrodes in buffer solutions containing glucose were utilized as scaffolds for direct electron transfer (DET)-type bioelectrocatalysis of bilirubin oxidase (BOD). The three-dimensional porous structure appeared to promote DET-type bioelectrocatalysis of BOD. The current density of DET-type bioelectrocatalysis of BOD increased with the formation of a three-dimensional porous structure at the beginning of anodization; however, it showed saturation characteristics with a further increase in the non-Faradaic current. Moreover, the glucose concentration during anodization affected the activity of DET-type bioelectrocatalysis of BOD. Scanning electron microscopy of the prepared porous Au electrodes showed that the structure of the electrode surface depended on the glucose concentration. Achieving a pore size distribution suitable for substrate transport and enzyme orientation seemed to be dependent on the glucose concentration.