LAUSR

Abstract An effective electrochemical sensing platform based on electrodeposited NiO nanoparticles and graphene oxide (GO) has been successfully developed for the simultaneous determination of uric acid (UA), dopamine (DA) and ascorbic acid (AA). The chemical composition and morphology characteristic of NiO/GO nanocomposite were investigated by X-ray diffraction, Raman spectroscopy and scanning electron microscopy. Furthermore, cyclic voltammetry and differential pulse voltammetry were applied to explore the electrochemical properties of the constructed electrode. As a result, the linear amperometric responses to UA, DA and AA were achieved in the concentration ranges of 2.0 ∼ 120, 2.0 ∼ 60 and 40 ∼ 700 μM with detection limits of 0.14, 0.10 and 5.50 μM, respectively. Moreover, the method functioned well on the determination of these three molecules in real urine samples with acceptable selectivity, favorable reproducibility and durable stability. The results suggested that this electrochemical sensing platform has potential application for the simultaneous detection of UA, DA and AA in biological samples.