LAUSR

The formation of fat, oil, and grease (FOG) deposits in sewers is a global challenge for the maintenance of sewer collection systems. Tons of FOG deposits (FDs) are removed from sewer systems every year and present an opportunity for increased methane production via anaerobic co-digestion with waste activated sludge (WAS) at water resource recovery facilities with existing anaerobic digesters. We hypothesized that FDs have higher biomethane potential than that of FOG (e.g., FOG collected in grease interceptors), because of the reduction of inhibition of long chain fatty acids due to saponification. In this study, substantially enhanced methane production was found in anaerobic co-digestion of WAS with FDs within the substrate to inoculum (S/I) ratio range of 0.25-1.2, and the maximum ultimate methane production (685.7 ± 24.1 mL/gVSadded, at S/I = 0.5) was 4.0 times higher than in the control (with WAS only) after 42 days of incubation. Although the lag phase period was longer in FD co-digestion (S/I = 0.5) than in FOG co-digestion (S/I = 0.5) under the same organic loading (gVS) and two times the COD loading, the daily methane production rate became higher after Day 15 in FD co-digestion. Significantly higher cumulative methane production (10.2%, p < 0.05) was obtained in FD co-digestion than in FOG co-digestion after 42-days. Microbial community analysis revealed higher levels of Geobacter in FD co-digestion, possibly suggesting a role for direct interspecies electron transfer (DIET) between Methanosaeta and Geobacter. This work provides fundamental insights supporting anaerobic co-digestion of FDs with WAS, demonstrating the advantages of FDs compared to FOG as co-substrate for enhanced biomethane recovery.