LAUSR

Anaerobic digestion is a well-established process that is applied to treat organic wastes and convert the carbon to valuable methane gas as a source of energy. The digestate that comes out as a by-product is of a great challenge due to its high nutrient content that can be toxic in case of improper disposal to the environment. Several attempts have been done to valorize this digestate. Digestate has been considered as an interesting medium to cultivate microalgae. The nutrients available in the digestate, mainly nitrogen and phosphorus, can be an interesting supplement for microalgae growth requirement. The main obstacles of using digestate as a medium to cultivate microalgae are the dark color and the high ammonium-nitrogen concentration. The focus of this review is to discuss in detail the major attempts in research to overcome inhibition and enhance microalgae cultivation in digestate. This review initially discussed the obstacles of digestate as a medium for microalgae cultivation. Different processes to overcome inhibition were discussed including dilution, supplying additional carbon source, favoring mixotrophic cultivation and pretreatment. More emphasis in this review was given to digestate pretreatment. Among the pretreatment methods, filtration, and centrifugation were of the most applied ones. These strategies were found to be effective for turbidity and chromaticity reduction. For ammonium nitrogen removal, ammonia stripping and biological pretreatment methods were found to play a vital role. Adsorption could work both ways depending on the material used. Combining different pretreatment methods as well as including selected microalgae stains were found interesting strategies to facilitate microalgae cultivation with no dilution. This study recommend that more study should investigate the optimization of microalgae cultivation in anaerobic digestate without the need for dilution.