LAUSR

Hair loss (alopecia) is a skin disease that affects the appearance. It has become a common concern in Chinese society. The classic Tibetan medicine book Jingzhu Materia Medica records that "the nut oil from Prunus mira(Tibetan name: ཁམབུ།) is used to cure loss of hair, eyebrows, beards, etc." but the clinical experience has not been explored. This paper takes nut oil from Prunus mira as its research subject. MATERIALS AND METHODS The content of the components in the nut oil from P. mira was determined by HPLC. The effects of different doses of nut oil on hair growth were studied by two hair removal methods(sodium sulfide and depilatory cream) in KM mice. Then select the group with obvious effect to determine the number of hair follicles, dermal thickness, determine mRNA and protein expression of β-catenin, GSK3β and Wnt10b in C57BL/6 mice. RESULTS The contents of α-tocopherol, β-sitosterol, Vitamin E, Oleic acid and linoleic acid in 10 different origins of nut oil from P. mira were determined by HPLC. The linearity reached 0.999. The RSD of precision, stability, repeatability, and sample recovery was less than 3%. The dose-effect relationship found that the diluted twice and four times oil promoted hair growth and the dose effect was positively correlated. Diluted twice the P. mira nut oil can accelerate hair follicles into the anagen, increase Wnt 10b mRNA expression, β-catenin mRNA and protein expression, and Gsk-3β protein expression. CONCLUSION This study improved the quality control of nut oil from P. mira and found that it has the effect of promoting hair growth in mice. The working mechanism may be related toWnt/β-catenin signaling pathway.