LAUSR

ETHNOPHARMACOLOGICAL RELEVANCE The extracts of Jerusalem thorn fruits (JT-FE) have been commonly used for the treatment of diabetes mellitus in Turkey. AIM OF THE STUDY In this study, it is aimed to investigate the effects of the JT-FE, prepared by decoction, on blood glucose, insulin and glycated haemoglobin levels of diabetic rats induced with streptozotocin (STZ). Hypoglycemic activity of the extracts was examined in streptozotocin-induced diabetic rats. MATERIALS AND METHODS For this purpose, pre-prandial blood sugar, insulin and glycated hemoglobin levels were measured. To investigate active substances that were responsible for the antidiabetic activity, phytochemical analysis was carried out with optimized and validated LC-MS/MS method using 53 phytochemicals in JT-FE. In addition, ICP-OES analysis was performed to determine the mineral content. RESULTS The findings of the study demonstrate that when insulin and JT-FE applied groups were compared with the diabetic control group, their blood sugar and glycated hemoglobin levels were seen to statistically decrease (p < 0,001). Morewer, When JT-FE treated groups were compared with insulin-treated groups, a statistically decrease (p < 0,05) in their levels was observed. On the other hand, it was also found that the increase in extract concentration didn't contribute significantly to antidiabetic activity. As a result of the phytochemical analysis, total of 31 different phenolic compounds were defined in JT-FE. The major components of JT-FE (as analyte/g extract) were; rutin (98753.4 ± 24.39 μg), catechin (58695.3 ± 12.971 μg), hesperidin (47445.2 ± 15.894 μg), quinic acid (38279.5 ± 14.239 μg) and malic acid (17536.8 ± 2.279 μg). In the mineral analysis we made; Sodium, calcium, magnesium and phosphorus elements were found at macro level, Zn and Cr3+ minerals were found at trace level. CONCLUSION Our findings show that JT-FE, prepared by decoction, is rich in phenolic and mineral content and strong in antihyperglycemic activity. That's why Jerusalem thorn fruits can be a useful antidiabetic phytotherapy agent.