LAUSR

Abstract Vitex negundo L. var. heterophylla (Franch.) Rehd. is widely accepted as a functional and medicinal food in China because of the presence of bioactive components which improve the physical condition. In the present study, the bioactive constituents, antioxidant, α -glucosidase and DPP-IV inhibitory activities of V. negundo var. heterophylla were investigated. Two new coumarin glycosides, vitexnegheteroins I-J ( 1 – 2 ), and two new iridoid glycosides, vitexnegheteroins K-L ( 3 – 4 ), were isolated from the leaves and seeds of V. negundo var. heterophylla , together with twenty known compounds. Seven compounds presented stronger antioxidant activities than l -ascorbic acid in both DPPH and ABTS assays. Among them, isoorientin-6″- O -caffeate ( 22 ) also exhibited more potent α -glucosidase inhibitory activity than acarbose with an IC 50 value of 0.24 ± 0.02 μM. These findings further revealed the bioactive components of this edible aromatic plant and disclosed the potency of it to be developed as new antioxidant and α -glucosidase inhibitory agents.