LAUSR

Abstract We investigated the effect of polyphenols extracted from M. angusticepes Peck (PMP) on D-galactosamine/lipopolysaccharide (D-GalN/LPS) induced acute hepatic failure (AHF) in mice. Results indicated that administration of PMP relieved the leakage of serum alanine aminotransferase (ALT) and aspartate aminotransferase (AST). The level of serum ALT was reduced in groups of P-H (800 mg PMP/kg BW) and P-L (400 mg PMP/kg BW) by 44.6% and 31.8%, respectively. RT-PCR and Western blot analyses showed that NF-E2-related factor 2 (Nrf2), PPARα, PPARγ gene and protein were up-regulated after injection D-GalN/LPS. In contrast, PMP pretreatment noticeably prevented D-GalN/LPS-induced hepatocyte apoptosis and steatosis. It suggested that PMP attenuated D-GalN/LPS-induced AHF in mice, which was likely attributed to the co-activation of Nrf2, PPARα, PPARγ and alleviating the oxidative stress reaction, reducing the body inflammatory response and regulating the lipid metabolism.