LAUSR

Abstract Endometrial cancer (EC) associated with endogenous hormone metabolism, obesity, diabetes, and inappropriate dietary habits has shown a rising trend in recent years. Various therapeutics methods including dietary approaches (vital phytochemicals) offer healthy and nontoxic strategies against cancer. To date, 6-shogaol as one of the antitumor phytochemicals remains unexplored against human endometrial cancer cells. In our study, 6-shogaol obtained from Zingiber officinale rhizomes separated and purified by HPLC, LC-MS/MS, and NMR respectively could inhibit Ishikawa cell proliferation at IC50 (24.91 μM) with the arrested cell cycle in the G2/M phase. Moreover, it promoted apoptosis, as a result, triggered ROS production, then activated key ER response biomarkers in Ishikawa cell associated with mitochondria, and ultimately regulated related genes and proteins in vitro and in vivo. Therefore, 6-shogaol can be used as a promising natural compound endowed with potential benefits for cancer treatment and/or prevention at large and EC in particular.