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Preparation and characterization of whey protein isolate-chlorophyll microcapsules by spray drying: Effect of WPI ratios on the physicochemical and antioxidant properties

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Abstract Whey protein isolate-chlorophyll (WPI-CH) microcapsules at different WPI ratios via spray drying were created to improve water solubility and biological stability of chlorophyll. Samples at a higher ratio of… Click to show full abstract

Abstract Whey protein isolate-chlorophyll (WPI-CH) microcapsules at different WPI ratios via spray drying were created to improve water solubility and biological stability of chlorophyll. Samples at a higher ratio of WPI resulted in a lower moisture content (1.14 ± 0.12% to 2.47 ± 0.09%), higher chlorophyll encapsulation efficiency (98.34 ± 0.03% to 99.76 ± 0.02%) and greater chlorophyll solubility (79.29 ± 0.07% to 82.24 ± 0.03%). The results of fluorescence and FT-IR spectra showed that the pyrrole ring of chlorophyll became bound to the hydrophobic residues of WPI leading to a change in the crystalline structure of chlorophyll, probably due to the formation of soluble complexes of WPI-CH. In addition, the antioxidant activity of fresh WPI-CH samples, and those stored for 6 months, increased by 19.75% and 18.51% respectively compared to WPI (p

Keywords: isolate chlorophyll; whey protein; chlorophyll; wpi; protein isolate; wpi ratios

Journal Title: Journal of Food Engineering
Year Published: 2020

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