LAUSR

OBJECTIVES The rapid spread of Klebsiella spp. is recognized as a major threat to public health owing to a rise in the number of both healthcare- and community acquired infections. Here we report the draft genome sequence of a high carbapenem-resistant strain Klebsiella quasipneumoniae subsp. quasipneumoniae Cln185 isolated from an HIV patient with pneumonia. METHODS Classical microbiological methods were applied to isolate and identify the strain, genomic DNA was sequenced using an Illumina HiSeq platform and the reads were de novo assembled into contigs using CLC Genomics Workbench. The assembled contigs was annotated and whole genome sequencing (WGS) analysis was performed. RESULTS WGS analysis revealed the genome comprised a circular chromosome of 5,406,774 bp with a G-C content of 57.73 %. Three important antibiotic resistance genes, IMP-38, OKP-B-6, and DHA-1, were detected. In addition, genes conferring resistance to aminoglycosides, macrolides, sulfonamide, fluoroquinolones, and tetracycline were also identified. CONCLUSION The draft genome sequence reported herein will lay the foundation for future research for antibiotic resistance mechanisms and pathogenic mechanisms in Klebsiella quasipneumoniae subsp. quasipneumoniae, and also will promote the comparative analysis with genomic features among different sources of clinically important multidrug resistance strains.