LAUSR

OBJECTIVES To determine the draft genome sequence and analyze the genetic features of a Staphylococcus pseudintermedius clinical isolate according to the main typing schemes available with a special focus on antibiotic resistance. METHODS The strain was isolated from a case of otitis externa in a dog. Its identity and pattern of antibiotic susceptibility were determined by an automated system. The genome has been sequenced using an Illumina platform. MLST, SCCmec typing, resistome, and mobile genetic elements were derived by comparative analysis using available specific databases. RESULTS S. pseudintermedius CAM1 isolate has a chromosome size of 2 652 610 bp. It showed a wide pattern of phenotypic resistance, comprising beta-lactams, macrolides and lincosamides, aminoglicosides, fluoroquinolones, tetracyclines, and trimethoprim-sulfamethoxazole. The genetic determinants of the underlying mechanisms have all been found by in silico analysis of the genome. mecA gene for methicillin resistance was harbored by the Vc type of the SCCmec. MLST of the strain was the st551. CONCLUSION By comparison with the MLST database of S. pseudintermedius and data from published molecular epidemiology studies, CAM1 is the first st551 strain recorded in Italy and, in the context of an already extremely wide antibiotic resistance pattern, it harbors also the tetK gene, whose prevalence is rare in MDR S. pseudintermedius.