LAUSR

This review aims to encourage the technical development of microbial biodiesel production from industrial-organic-wastes-derived volatile fatty acids (VFAs). To this end, this article summarizes the current status of several key technical steps during microbial biodiesel production, including (1) acidogenic fermentation of bio-wastes for VFA collection, (2) lipid accumulation in oleaginous microorganisms, (3) microbial lipid extraction, (4) transesterification of microbial lipids into crude biodiesel, and (5) crude biodiesel purification. The emerging membrane-based bioprocesses such as electrodialysis, forward osmosis and membrane distillation, are promising approaches as they could help tackle technical challenges related to the separation and recovery of VFAs from the fermentation broth. The genetic engineering and metabolic engineering approaches could be applied to design microbial species with higher lipid productivity and rapid growth rate for enhanced fatty acids synthesis. The enhanced in situ transesterification technologies aided by microwave, ultrasound and supercritical solvents are also recommended for future research. Technical limitations and cost-effectiveness of microbial biodiesel production from bio-wastes are also discussed, in regard to its potential industrial development. Based on the overview on microbial biodiesel technologies, an integrated biodiesel production line incorporating all the critical technical steps is proposed for unified management and continuous optimization for highly efficient biodiesel production.