LAUSR

Abstract Purpose Study the effect of AiQingHua (AQH) oil on auricle microcirculation disorder mice caused by epinephrine and the effect of promote hair regeneration in mice with pathological alopecia caused by testosterone. Methods: Model of auricle microcirculation disorder in mice induced by intravenous injection of epinephrine hydrochloride (10 mg/kg), observed the auricle capillary vein and arteriole diameter, blood flow velocity and the amount of capillary opening before and 2 min after injection. The model of alopecia was established in mice induced by testosterone, the mold-making process was also externally applied to the drug for 21 consecutive days. On the day when the hair began to grow, the hair growth status was graded every four days. The serum levels of testosterone (T) and estradiol (E2) were measured on 21 d. The alopecia part of the skin was taken and the pathological changes were observed. Results: Compared with the blank group, minoxidil tincture group and the large and small dosage of AQH oil group can expand the diameter of auricle capillary vein and arteriole, increase the number of capillary opening and improve blood flow of mice with auricle microcirculation disorder. All mice in each group had hair growth from the 8th day of administration. Hair growth score in each administration group was superior to the model group on the 12th day, the 16th day and the 20th day. Each administration group can reduce the serum T and E2 level of mice with hair loss to different degrees, and reduce the ratio of T/E2, and improve the number of hair follicles, sebaceous glands and sweat glands in hair loss area. Conclusion: AQH oil can improve the microcirculation of mice and promote hair regeneration in mice with hair loss.