LAUSR

Bacteria employ several mechanisms, and most notably secretion systems, to translocate effectors from the cytoplasm to the extracellular environment or the cell surface. Pseudomonas aeruginosa widely employs secretion machineries such as the Type III secretion system (T3SS) to support virulence and cytotoxicity. However, recently identified P. aeruginosa strains that do not express T3SS have been shown to express ExlA, an exolysin translocated through a Two Partner Secretion System (TPS), and are the causative agents of severe lung hemorrhage. Sequence predictions of ExlA indicate filamentous haemagglutinin (FHA-2) domains as the prevalent features, followed by a C-terminal domain with no known homologs. In this work we have addressed the mechanism employed by ExlA to target membrane bilayers by using NMR, small angle X-ray scattering, atomic force microscopy, and cellular infection techniques. We show that the C-terminal domain of ExlA displays a 'molten globule-like' fold that punctures small holes into membranes composed of negatively-charged lipids, while other domains could play a lesser role in target recognition. In addition, epithelial cells infected with P. aeruginosa strains expressing different ExlA variants allow localization of the toxin to lipid rafts. ExlA homologs have been identified in numerous bacterial strains, indicating that lipid bilayer destruction is an effective strategy employed by bacteria to establish interactions with multiple hosts.