LAUSR

PURPOSE To evaluate the microshear bond strength (μSBS) to deep (DD) or superficial (SD) dentin (μSBS) overtime, nanoleakage (AG%), degree of conversion (DC%), water sorption (WSp), and solubility (WSl) of an adhesive system [Adper Single Bond 2(ASB)] containing matrix metalloproteinases (MMP) inhibitors [GM1489 (ASB-GM), Batimastat (ASB-BAT), or Chlorhexidine diacetate (ASB-CHX)]. ASB without inhibitor was used as control (CONTROL). MATERIALS AND METHODS WSp and WSL were calculated based on ISO4049. DC% was analyzed using FT-IR spectroscopy. Dentin discs were used for μSBS evaluation. For AG%, resin-dentin beams were analyzed under scanning electronic microscopy. Data were analyzed using three-way ANOVA (AG% and μSBS) or ANOVA (DC%, WSp, WSl) and Tukey's HSD test. RESULTS ASB-CHX presented the lowest DC%, lowest WSp, and highest WSl. ASB-GM reached the highest immediate μSBS in SD, only different from ASB-CHX. In DD, ASB-BAT and ASB-GM had the highest μSBS, statistically different from ASB-CHX. After twelve months, ASB-GM and ASB-BAT presented higher μSBS in SD when compared to CONTROL and ASB-CHX. In DD, ASB-GM reached the highest value, which was statistically different from CONTROL and ASB-CHX. CONTROL at both dentin depths and ASB-CHX at DD did not maintain bond stability. In SD after 12 months, ASB-BAT and ASB-GM decreased AG%. In DD, only ASB-GM reduced AG%. CONCLUSION The ASB containing Batimastat and GM1489 maintained resin-dentin bond stability after 12 months for both dentin depths, without jeopardizing WSp, WSl, or DC% of the adhesive system. The ASB-GM presented greater μSBS after 12 months when compared with ASB. CLINICAL SIGNIFICANCE Batimastat and GM1489 could be suitable for inclusion as an MMP-inhibitor into Single Bond to improve the bond stability to superficial and deep dentin, without jeopardize the physic-mechanical proprieties.