LAUSR

Study Objective Profiling of miRNA and mRNA in eutopic and ectopic endometrial tissues in patients with endometrioma. Design A prospective cohort study. Setting This study was conducted at the Department of Operative Gynecology of V.I. Kulakov National Medical Center for Obstetrics, Gynecology, and Perinatology. Patients or Participants This study included 136 women of reproductive age from 18 to 38 years with endometriomas (OE). Interventions Determination of the differences in the expression of miRNA and mRNA in the tissues of OE (EcE) and eutopic endometrium (EuE); bioinformatic analysis to evaluate the role of differentially expressed RNA in the pathogenesis of OE; analysis of microRNA transcriptome (TRA) performed by the sequencing method. Measurements and Main Results TRA revealed significant differences in the expression of miRNA and mRNA in EcE and EuE. It was found that the expression of miRNA and mRNA depends on the type of ovarian reserve (OVR). Lists of target genes were obtained for identified miRNAs were involvement of regulated target genes in the processes of proliferation, in migration and invasion, and in the key cascades of intracellular signaling and inflammatory processes and in the processes of DNA repair and regulation of the mitotic cycle, which indicates the genotoxic effect of pathogenetic factors acting in the tissues of OE and on adjacent ovarian tissues. Conclusion The data obtained suggest that the change in the expression of microRNA in the tissues of the ectopic endometrium can make a significant contribution to the development of endometriosis and the associated reduction of the OVR.