LAUSR

BACKGROUND Electrical probes have been widely used for recording single-unit spike activity and local field potentials (LFPs) in brain regions. However, setting up an easily-assembled large-scale recording in multiple brain regions for long-term and stable neural activity monitoring is still a hard task. NEW METHOD We established a novel 3D-printed multi-drive system with high-density (up to 256 channels) tetrodes/grid electrodes that enables us to record cortical and subcortical brain regions in freely behaving animals. RESULTS In this paper, we described the design and fabrication of this system in detail. By using this system, we obtained successful recording on both spikes and LFPs from seven distinct brain regions that are related to memory function. COMPARISION WITH EXISTING METHODS The low cost, large-scale electrodes with small size and flexible 3D-printed design of the system allow us to implant assembled tetrodes or grid electrodes into multiple targeting brain areas. CONCLUSIONS The 3D-printed large-scale multi-drive platform we described here may serve as a powerful new tool for future studies of brain circuitry functions.