LAUSR

INTRODUCTION This study evaluated photodynamic therapy (PDT) and photobiomodulation therapy (PBM) as adjuncts to pulp revascularization using cultures of apical papilla cells (APCs) and endothelial cells (HUVECs). METHODS The root canal and apical foramen of two mandibular first premolars were enlarged to simulate immature teeth. The canal of one tooth was filled with 1 mL of 0.005% methylene blue (MB). After that, the canals of both teeth were irrigated with 20 mL of 1.5% sodium hypochlorite (NaOCl) and 20 mL of 17% ethylene diaminetetraacetic acid (EDTA). The resulting solutions were diluted in cell culture media at a concentration of 0.5% (0.5% MB+NaOCl+EDTA; 0.5% NaOCl+EDTA). After PDT (0.5% MB+NaOCl+EDTA+PDT) and PBM (0.5% NaOCl+EDTA+PBM) applications, effects were evaluated to determine cytotoxicity, polarity index, APCs migration and HUVECs sprouting, and results were compared with those of their controls (solutions without laser application). Cell culture media (CT) was also used as a control. Data were analyzed using one-way ANOVA and the Tukey post hoc test (P≤0.05). RESULTS PDT and PBM promoted greater APCs viability than their controls, and PDT had greater cell viability than CT (P<0.05). All protocols reduced APCs migration when compared with CT (P<0.05). HUVECs sprouts grown out of spheroids in PBM had a greater ratio area than its control (P≤0.01), and PDT ratio of spheroid area was similar to that of its control (P>0.05). CONCLUSIONS PBM and PDT seem to be potentially effective adjuncts to revascularization in non-vital immature teeth.