LAUSR

INTRODUCTION The purpose of this study was to observe the release of vascular endothelial growth factor (VEGF) after conditioning with etidronic acid, 1-hydroxyethylidene-1, 1-bisphosphonate (HEDP) and phytic acid, inositol hexakisphosphate (IP6) in comparison with EDTA on human dentine disks and cylinders. METHODS Human dentine disks were disinfected and prepared by standardized method. Seventeen percent EDTA, 9% HEDP, 1% IP6 and distilled water (5 minutes) were used to immerse dentine disks. The dentine cylinders were prepared by following disinfection and standardized preparation method to achieve a truncated cone shaped canal with 1mm open apex. The dentine cylinder samples were irrigated with sodium hypochlorite (NaOCl) (5 mL/5 minutes) and then rinsed with 17% EDTA, 9% HEDP, 1% IP6 and distilled water (5 mL/5 minutes). Enzyme linked Immunosorbent Essay (ELISA) was performed to measure VEGF release. One way analysis of variance (ANOVA) was used to compare the mean release of VEGF between study groups and controls at a significance level of 0.05. A post hoc Tukey test was used for multiple comparisons between study groups. RESULTS Among conditioners, HEDP released more VEGF from both disks and cylinders. In cylinders, VEGF release by HEDP was significantly greater than other conditioners. Whereas in disks the release of VEGF was similar with all conditioners. CONCLUSIONS The release of VEGF by 9% HEDP and 1% IP6 was comparable to 17% EDTA from dentine disk, however, HEDP demonstrated more release from dentine cylinder than EDTA and IP6.