LAUSR

OBJECTIVE This study aimed to evaluate the influence of oral supplementation with benfotiamine on oxidative stress in the liver, heart and muscles of endurance-trained mice. METHODS Twenty-five male BALB/c mice were allocated to the following treatment groups: standard diet and sedentary activity (Sta-Sed), benfotiamine-supplemented diet and sedentary activity (Ben-Sed), standard diet and training activity (Sta-Tr) and benfotiamine-supplemented diet and training activity (Ben-Tr). The training comprised 6 weeks of endurance swimming training. The concentration of thiobarbituric acid reactive substances (TBARS), carbonylated proteins, total thiols and non-protein thiols was analyzed in the liver, heart and tibialis anterior muscle. RESULTS In the muscle, TBARS concentration in the Sta-Sed group was higher than that in other groups; in the heart, TBARS concentration in the Sta-Sed and Ben-Tr groups was higher than that in the Ben-Sed group. The carbonyl content of the muscle tissues was higher in the Sta-Sed group than in both supplemented groups. In liver, the carbonyl content was lower in the Ben-Sed group than in the Sta-Sed group. The level of total thiols was lower in the Ben-Sed group than in the Sta-Tr group. In the heart, the level of total thiols was higher in the Ben-Sed group than in the Ben-Tr group. The concentration of non-protein thiols in the muscle was higher in the Ben-Sed group than in the Ben-Tr group, whereas in the heart, concentration of non-protein thiols of Sta-Tr group was lower than that of Sta-Sed group. CONCLUSION The results show that benfotiamine is an efficient antioxidant for the anterior tibialis muscle and heart; however, swimming training did not alter redox status.