LAUSR

An enantioselective study of alminoprofen (AMF) in rat plasma was performed by a rapid, specific and sensitive chiral liquid chromatography-tandem mass spectrometry (LCMS/MS) method. A homemade β-cyclodextrin (β-CD) derivatized based chiral column and a commercial polysaccharide type chiral column were selected and evaluated. The former one per-4-chlorophenylcarbamate-β-CD bonded chiral stationary phase (CSP) which was synthesized in our lab produced the enhanced enantioselective separation. The dextro-ketoprofen (d-KTF) was selected as the internal standard (IS). The approach was linear in the concentration ranges of 1.00-20000.0 ng mL-1 (r2≥0.99) for each enantiomer. The mean recoveries of R-(-)- and S-(+)-AMF at three spiked levels of 2.00, 1000.0 and 16000.0 ng mL-1 ranged from 92.0 %-96.1 %, and the intra-day and inter-day relative standard deviations (RSDs) were within 8.9 %. The lower limit of quantification (LLOQ) values of R-(-)- and S-(+)-AMF in rat plasma matrices were both 1.00 ng mL-1. The established method was successfully applied to a stereoselective pharmacokinetic study of AMF enantiomers in rat plasma following oral administration. The pharmacokinetic results revealed that S-(+)-AMF displayed prominently higher Cmax and AUC values than R-(-)-enantiomer.