Highlights • Isothermal assays representative of RT-LAMP and RT-RPA were compared against rRT-PCR.• Nine sample preparation methods were evaluated across all assays.• RT-LAMP can detect FMDV in multiple sample types… Click to show full abstract
Highlights • Isothermal assays representative of RT-LAMP and RT-RPA were compared against rRT-PCR.• Nine sample preparation methods were evaluated across all assays.• RT-LAMP can detect FMDV in multiple sample types using simple preparation techniques.• RT-LAMP can detect FMDV across a large diagnostic detection window.
               
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