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Defining the relative performance of isothermal assays that can be used for rapid and sensitive detection of foot-and-mouth disease virus

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Highlights • Isothermal assays representative of RT-LAMP and RT-RPA were compared against rRT-PCR.• Nine sample preparation methods were evaluated across all assays.• RT-LAMP can detect FMDV in multiple sample types… Click to show full abstract

Highlights • Isothermal assays representative of RT-LAMP and RT-RPA were compared against rRT-PCR.• Nine sample preparation methods were evaluated across all assays.• RT-LAMP can detect FMDV in multiple sample types using simple preparation techniques.• RT-LAMP can detect FMDV across a large diagnostic detection window.

Keywords: detection; defining relative; assays used; isothermal assays; performance isothermal; relative performance

Journal Title: Journal of Virological Methods
Year Published: 2017

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