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Ion torrent-based nasopharyngeal swab metatranscriptomics in COVID-19

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Abstract Herein, we describe the detection of a SARS-CoV-2 genome through metatranscriptome next-generation sequencing directly from the nasopharyngeal swab of a suspected case of local transmission of Covid-19, in Brazil.… Click to show full abstract

Abstract Herein, we describe the detection of a SARS-CoV-2 genome through metatranscriptome next-generation sequencing directly from the nasopharyngeal swab of a suspected case of local transmission of Covid-19, in Brazil. Depletion of human ribosomal RNA and use of an optimized in-house developed bioinformatics strategy contributed to successful detection of the virus.

Keywords: nasopharyngeal swab; metatranscriptomics covid; swab metatranscriptomics; based nasopharyngeal; torrent based; ion torrent

Journal Title: Journal of Virological Methods
Year Published: 2020

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