LAUSR

Abstract F4- and F18-positive enterotoxigenic Escherichia coli (ETEC) are well-known pathogens able to cause a severe swine disease with a worldwide economically importance in pig production. Vaccination of piglets with an oral live bivalent F4/F18 Escherichia coli vaccine has shown some promise and efficacy results in preventing ETEC post weaning diarrhoea (PWD); however, currently, there is a lack of knowledge on its effect on the intestinal microbial community. Furthermore, is not known if the effect of vaccination on gut eubiosis can vary according to pig genetic susceptibility to ETEC F4 and F18. The aim of this study is to evaluate the effect of an E. coli bivalent vaccine and the potential influence of the host genetic susceptibility to ETEC growth performance and faecal microbial profile of healthy piglets around weaning. A total of 288 healthy piglets at 26 days of age (d0; weaning) were divided into two different groups balanced for body weight: i) vaccine (VAX: 6.33 kg) and control (CO: 6.45 kg). At d0 VAX group was orally inoculated with 2 mL of live non-pathogenic E. coli O8:K87 (F4ac-positive) and O141:K94 (F18ac positive) vaccine (Coliprotec F4/F18; Prevtec Microbia an Elanco Company), while CO was orally inoculated with 2 mL of sterile water. At d0 and 18 days later (d18), piglets were individually weighed, and a faecal sample was collected for microbial profile of V3-V4 regions of the 16S rRNA gene using MiSeq Illumina platform. Bristles were collected to determine the genetic ETEC susceptibility by genotyping analysis of MUC4 and FUT1 polymorphisms. No effect of vaccination and genetic susceptibility was observed on growth performance. The vaccination modulated the faecal microbial composition, reducing the alpha diversity indices (P