LAUSR

Abstract The efficient utilization of rice bran is severely impeded because of the rapid rancidity caused by rice bran lipase, so the investigation of rice bran lipase characteristic and catalytic mechanism was urgently required. The extracted crude proteins from rice bran were purified to obtain two lipases with 16.31 times of purification fold and 10.64% of purification yield. The molecular masses of these two homologous lipases were 34929.00 Da and 34072.00 Da, respectively. Rice bran lipase showed highest catalytic activity at 40.00 °C, pH 7.50 and held good stability below 50.00 °C and at pH 7.00–7.50. Most metal ions (except Na+ and Co+) and organic solvents had significant inhibition effect (P