LAUSR

Abstract To acquire stable non-covalent functionalized carbon nanotubes with good dispersion and biocompatibility, cinnamaldehyde-chitosan conjugates (CA-CS conjugates) were synthesized to modify carbon nanotubes. The functionalized carbon nanotubes (CA-CS-CNTs) were characterized by FTIR, TGA and TEM. CA-CS-CNTs could be dispersed well in water, and the loading of CA-CS conjugates on carbon nanotubes was about 23%. CA-CS-CNTs were further extended to immobilize porcine pancreatic lipase (PPL), and the successful attachment of PPL onto CA-CS-CNTs was revealed by the result of FTIR. The lipase loading and relative lipase hydrolysis activity of immobilized PPL (CA-CS-CNTs-PPL) reached 248 mg/g and 8064 U/g, respectively. CA-CS-CNTs-PPL possessed good thermostability, and maintained 69% of enzyme activity after reusing 7 times. Additionally, characteristic flavor of strong aromatic liquors was prepared using CA-CS-CNTs-PPL as biocatalysts, and the highest yield of 88% was observed. CA-CS-CNTs-PPL possessed positive properties, which could be expected to become low-cost and useful biocatalysts.