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De novo transcriptome assembly of the amphipod Gammarus chevreuxi exposed to chronic hypoxia

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Abstract Environmental hypoxia is becoming more prevalent in aquatic environments due to eutrophication and climate change. While the ecological and physiological responses of marine animals to hypoxia have received considerable… Click to show full abstract

Abstract Environmental hypoxia is becoming more prevalent in aquatic environments due to eutrophication and climate change. While the ecological and physiological responses of marine animals to hypoxia have received considerable attention, the molecular responses remain largely undetermined. We have assembled a transcriptome for the brackishwater amphipod, Gammarus chevreuxi , exposed to three different levels of environmental oxygen (100, 40 and 20% air saturation). Sequencing using Illumina HiSeq 2000 produced 227.1 M reads which were assembled into 291,934 contigs corresponding to 218,558 genes. The assembled transcriptome provides a valuable resource to explore the molecular mechanisms underpinning responses to chronic hypoxia in an ecologically-important aquatic invertebrate.

Keywords: chronic hypoxia; novo transcriptome; gammarus chevreuxi; hypoxia; chevreuxi exposed; amphipod gammarus

Journal Title: Marine Genomics
Year Published: 2017

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