LAUSR

Abstract The goal of this study is to construct bone scaffolds with neuroregulatory function. The scaffolds were prepared using bioglass (BG) powder, collagen (COL) solution, phosphatidylserine (PS) and ophiopogonin loaded COL microspheres as the main components. Combinatorial processing techniques involving chemical-crosslinking and freeze-drying were used. The resultant scaffold constructs were characterized in terms of their morphology, drug release kinetics, pharmacological and biological activities for osteoblast and neurocyte. The microporphology observation showed that the scaffolds had highly interconnected pores, favorable drug release kinetics in view of low initial release and slow average release rate. Moreover, cell studies showed that the involvement of ophiopogonin contributed to the proliferation and mineralization of MC3T3-E1 cells, and the neurite outgrowth of neuron. The drug-loaded composite scaffolds may therefore be a potential replacement in bone-tissue engineering.