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Assessment of NS1 gene-specific real time quantitative TaqMan PCR for the detection of Human Bocavirus in respiratory samples.

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Human Bocaviruses (HBoV) were associated with respiratory diseases. Here, we assessed a TaqMan®-based PCR for the detection of all four HBoV subtype infections with a sensitivity up to 15 copies/reaction.… Click to show full abstract

Human Bocaviruses (HBoV) were associated with respiratory diseases. Here, we assessed a TaqMan®-based PCR for the detection of all four HBoV subtype infections with a sensitivity up to 15 copies/reaction. To evaluate this assay on clinical samples, 178 nasopharyngeal aspirate specimens from pediatric cases were analyzed and HBoV genome was detected in 13 out of 178 patients with a viral load range between 1.6 × 103 and 9.4 × 107 copies/ml. These results indicated that this method could be used as an alternative technique for the diagnosis of HBoV infection.

Keywords: ns1 gene; taqman; assessment ns1; pcr detection; respiratory

Journal Title: Molecular and cellular probes
Year Published: 2017

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