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Immunohistochemical amplification of mCherry fusion protein is necessary for proper visualization

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Highlights • Virally expressed fluorescent reporter mCherry fusion protein does not adequately demonstrate its presence in fixed tissue.• Immunohistochemical amplification of mCherry can rescue this deficit. Click to show full abstract

Highlights • Virally expressed fluorescent reporter mCherry fusion protein does not adequately demonstrate its presence in fixed tissue.• Immunohistochemical amplification of mCherry can rescue this deficit.

Keywords: amplification mcherry; immunohistochemical amplification; mcherry fusion; mcherry; fusion protein

Journal Title: MethodsX
Year Published: 2020

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