LAUSR

Abstract In this work, a rapid and sensitive paper-based analytical device (PAD) method was developed for screening detection of total tetracyclines (TCs) in environment water. TCs form firstly a narrow stacking band on a paper channel by field amplification stacking, and then the band was visualized by fluorescent imaging which was induced by an ultraviolet light-emitting diode (UV LED). The image was acquired by a smartphone camera for quantitative analysis. Under optimal conditions, the sensitivity of this portable PAD system was 250 times enhanced within 2 min by online electrokinetic stacking and linear response was demonstrated in the range of 5–80 ng mL−1(R2 = 0.997) with detection limit of 4.5 ng mL−1. The applicability was demonstrated by detection of total TCs in environmental water with recovery ranging from 87 to 112% (RSD = 4.1–16.3%).