LAUSR

Abstract To develop a method to measure simultaneously the concentration of BTEX (benzene, toluene, ethylbenzene, m/p-xylene, o-xylene) in human whole blood with Purge and Trap-Gas Chromatography-Mass Spectrometry. The conditions of purge and trap were optimized, so that the BTEX in human whole blood could be enriched and desorbed, and then characteristic ions could be selected with gas chromatography–mass spectrometry combined with isotope internal standard for qualitative and quantitative analysis. After the parameters of purge and trap method were optimized, the BTEX in human whole blood could be simultaneously enriched, desorbed and measured accurately. The detection limit with this method ranges from 0.001 μg/L to 0.004 μg/L. The Linear range is from 0.021 μg/L to 10.99 μg/L. The recovery rate of low concentration (0.687 μg/L) solution is in the range of 79.21% to 85.92%; the recovery rate of high concentration (2.747 μg/L) solution is in the range of 93.21% to 102.07%. The analysis of the same sample is repeated for six times with its relative standard deviation ranging from 2.77% to 5.40%. With simple operation, short analysis period, good separation as well as required accuracy and precision, this method could analysis of BTEX concentration in human whole blood.