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Development of an Eco-friendly HPLC method for the simultaneous determination of three benzodiazepines using green mobile phase

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Abstract The analysis of the class of benzodiazepines (BDZs) is of a great importance on the levels of forensic, clinical and pharmaceutical uses. The study is focusing on the separation… Click to show full abstract

Abstract The analysis of the class of benzodiazepines (BDZs) is of a great importance on the levels of forensic, clinical and pharmaceutical uses. The study is focusing on the separation of three BDZs which are diazepam, clonazepam, and bromazepam using a green micellar mobile phase. It was found that the non-ionic surfactant Brij-35 can replace the organic solvent, as it decreases the retention time significantly and contributes to environmentally friendly or Eco-friendly separation procedures. The study was achieved on monolithic C18 column. Combination of sodium dodecyl sulphate (SDS): polyoxyethylene(23)lauryl ether (Brij-35) in a ratio of 50:50% v/v, was used as a mobile phase. The UV detection was set at 240 nm at a flow rate of 1.0 mL/min. The separation of diazepam, clonazepam and bromazepam was achieved in less than ten minutes even though there is no organic modifier in the mobile phase. A detailed study for the chromatographic parameters in this micellar system is carried out, in addition to a comparison with that achieved with other systems containing a single surfactant with low percent of organic modifier or other traditional mobile phase systems. Additionally, assessment of the greenness of the method is carried out using analytical eco-scale and the developed micellar HPLC method was proved to be an excellent green method for the analysis of the three drugs.

Keywords: mobile phase; eco friendly; using green; hplc method; phase

Journal Title: Microchemical Journal
Year Published: 2019

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