LAUSR

Abstract Antibiotics play an important role in disease prevention and as a growth promoter in the veterinary field. Animals derived foods holding antibiotic residues above the maximum residue limits can contribute as a hazardous effect for human health. In the current study, a BlaR-CTD mutant protein I188K/S19C/G24C enzyme-linked receptor assay (ELRA) was developed for the screening of 40 beta-lactams antibiotics in 12 edible animal tissues and in the milk sample. The IC50 for 40 drugs were ranged from 0.13 to 307.62 μg.L−1. The limit of detection (LOD) in tissues and milk samples were ranged from 0.01 to 110.2 μg.kg−1, and 0.07 to 194.64 μg.L−1 respectively. The evaluated average recovery rates in muscle, liver, kidney, and milk were ranged from 68.09 to 117.61%, 67.58 to 117.84%, 64.0 to 117.03%, and 68.08 to 116.06% respectively. The recovery rate in the entire sample examined in this study ranged from 60 to 120%, with co-efficient of variations below 15%. The applied ELRA showed a good correlation with that of LC-MS/MS. This study for the first time offers a simple and high throughput assay for the screening of 40 beta-lactams in 12 animal tissues and in milk sample, this assay was based on mutant protein I188K/S19C/G24C, with a wide range of high affinity and stability to beta-lactams antibiotics, exhibiting the potential to be commercially used.