LAUSR

Abstract The high price of the immunoaffinity column (IAC) has limited its broad application in the cleanup of analytes from complex matrices. This study aimed to reduce practical cost by regenerating IACs for reuse in cleaning up and purifying aflatoxin (AF) B1, B2, G1 and G2 from medicinal and edible malt samples, followed by high-performance liquid chromatography coupled with photochemical derivatization and fluorescence detection (HPLC-PCD-FLD). The IACs were prepared in advance by coupling anti-AFs specific antibodies (Abs) on a solid phase carrier. After each use, the IACs were washed immediately with phosphate-buffered saline (PBS) and pure water, respectively, and then were filled with PBS as the preservation solvent for storage at 4 °C overnight to recover the binding activity of the immobilized Abs for the regeneration and reuse in the next day until the recovery rate was lower than 70%. Results showed that the developed HPLC-PCD-FLD method displayed good linearity in wide concentration range with the limit of detection (LOD) and quantification (LOQ) of 0.3125–0.5 ng/mL and 1–1.5 ng/mL for the four aflatoxins, as well as excellent accuracy and precision with relative standard deviations (RSDs)