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A split-marker system for targeted gene deletion was developed for the model grass endophytic fungus Epichloë festucae. Compared to the conventional system that yields up to 25% homologous recombinants, the… Click to show full abstract
A split-marker system for targeted gene deletion was developed for the model grass endophytic fungus Epichloë festucae. Compared to the conventional system that yields up to 25% homologous recombinants, the method resulted in 33-74% targeted deletions in E. festucae using as little as 1.5kb of targeting sequence.
Keywords:
marker system;
system;
festucae using;
epichlo festucae;
split marker
Journal Title: Journal of microbiological methods
Year Published: 2017
Link to full text (if available)
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Journal Title: Journal of microbiological methods
Year Published: 2017
Link to full text (if available)
Share on Social Media: Sign Up to like & get
recommendations!