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Identification of heat resistant Escherichia coli by qPCR for the locus of heat resistance.

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Three qPCR assays targeting the locus of heat resistance to identify heat resistant clinical Escherichia coli isolates are described. Of 613 isolates, 3 (0.5%) possessed the locus. The assays are… Click to show full abstract

Three qPCR assays targeting the locus of heat resistance to identify heat resistant clinical Escherichia coli isolates are described. Of 613 isolates, 3 (0.5%) possessed the locus. The assays are a rapid, highly sensitive and specific alternative to screening by heat shock and can be used in food safety surveillance.

Keywords: heat resistance; escherichia coli; heat resistant; locus heat; heat; locus

Journal Title: Journal of microbiological methods
Year Published: 2017

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