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A development and an improvement of selectable markers in Pleurotus ostreatus transformation.

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Pleurotus ostreatus was transformed using the nourseothricin-resistant gene for the first time. The transformation efficiency was 1.3±0.6transformants/μg plasmid DNA. In addition, the transformation efficiency of the bialaphos-resistant gene was increased… Click to show full abstract

Pleurotus ostreatus was transformed using the nourseothricin-resistant gene for the first time. The transformation efficiency was 1.3±0.6transformants/μg plasmid DNA. In addition, the transformation efficiency of the bialaphos-resistant gene was increased to 26.7±11.5transformants/μg plasmid DNA.

Keywords: pleurotus ostreatus; markers pleurotus; development improvement; transformation; selectable markers; improvement selectable

Journal Title: Journal of microbiological methods
Year Published: 2017

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