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A flow-cytometric method to evaluate eosinophil-mediated uptake of probiotic Lactobacillus reuteri.

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Eosinophils are resident leukocytes of gut mucosa. Here we present a combined flow cytometric-antibiotic protection assay to identify mouse eosinophils capable of bacterial uptake, specifically, Gram-positive Lactobacillus reuteri, in studies… Click to show full abstract

Eosinophils are resident leukocytes of gut mucosa. Here we present a combined flow cytometric-antibiotic protection assay to identify mouse eosinophils capable of bacterial uptake, specifically, Gram-positive Lactobacillus reuteri, in studies performed ex vivo. The assay may be adapted for use in vivo.

Keywords: lactobacillus reuteri; cytometric method; flow cytometric; method evaluate

Journal Title: Journal of microbiological methods
Year Published: 2017

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