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We report here on EYK1, encoding erythrulose kinase, as an efficient catabolic selectable marker for genome editing in Y. lipolytica. Compared to auxotrophic markers, EYK1 increases the growth rate of… Click to show full abstract
We report here on EYK1, encoding erythrulose kinase, as an efficient catabolic selectable marker for genome editing in Y. lipolytica. Compared to auxotrophic markers, EYK1 increases the growth rate of transformants and allows improved efficiency of transformation. The utility of the marker EYK1 in a replicative vector was also demonstrated.
Keywords:
selectable marker;
erythrulose kinase;
catabolic selectable;
encoding erythrulose;
marker;
eyk1 encoding
Journal Title: Journal of microbiological methods
Year Published: 2017
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Journal Title: Journal of microbiological methods
Year Published: 2017
Link to full text (if available)
Share on Social Media: Sign Up to like & get
recommendations!