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A multiplex-PCR (mPCR) assay was designed with species-specific primers which generate amplicons of 226bp, 434bp and 106bp for differentiating the species C. striatum, C. amycolatum, and C. xerosis, respectively. mPCR… Click to show full abstract
A multiplex-PCR (mPCR) assay was designed with species-specific primers which generate amplicons of 226bp, 434bp and 106bp for differentiating the species C. striatum, C. amycolatum, and C. xerosis, respectively. mPCR results were 100% in agreement with identifications achieved by 16S rRNA and rpoB gene sequencing and by VITEK-MS.
Keywords:
species specific;
corynebacterium;
multiplex pcr;
xerosis;
specific primers
Journal Title: Journal of microbiological methods
Year Published: 2017
Link to full text (if available)
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Journal Title: Journal of microbiological methods
Year Published: 2017
Link to full text (if available)
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recommendations!