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A high throughput methodology for susceptibility testing of Mycobacterium tuberculosis isolates.

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MICs for eleven anti-TB drugs with M. tuberculosis isolates were obtained by means of agar dilution with multi-point inoculation. The results were compared with classic agar dilution and the MTT… Click to show full abstract

MICs for eleven anti-TB drugs with M. tuberculosis isolates were obtained by means of agar dilution with multi-point inoculation. The results were compared with classic agar dilution and the MTT assay. The multi-point inoculation method was reproducible with all drugs and correlated with classic agar dilution and MTT assay. This methodology can be used for routine breakpoint drug susceptibility testing (DST) and for MIC determination.

Keywords: methodology; susceptibility testing; agar dilution; tuberculosis isolates

Journal Title: Journal of microbiological methods
Year Published: 2018

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