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Quantifying transcription factor–DNA binding in live mouse embryos with photoactivatable fluorescence correlation spectroscopy (paFCS)

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ventral nerve cord to explore the role of cell-cell and tissue-tissue interactions. We use quantitative image analysis to extract the dynamic changes in the ventral nerve cord shape along its… Click to show full abstract

ventral nerve cord to explore the role of cell-cell and tissue-tissue interactions. We use quantitative image analysis to extract the dynamic changes in the ventral nerve cord shape along its entire length. We find that the ventral nerve cord does not uniformly condense, but has specific regions of high contractility which alter with developmental time. Finally, we incorporate these results within a mathematical model to understand this complex and dynamic organogenesis process.

Keywords: factor dna; nerve cord; quantifying transcription; ventral nerve; transcription factor; spectroscopy

Journal Title: Mechanisms of Development
Year Published: 2017

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