LAUSR

to prespore cells in slug (Mohamed et al.,2015). Sequence analysis of this region revealed canonical prespore specific activators including G-rich repeat element (GRE), Powell Coffman 1(PC1) Powell Coffman 2 (PC2) pair and New element at positions -988/-979, -1013/-996 and -1424/-1417 respectively. Three promoter specific CA rich elements (CAEs) at -578/-568(CAE1), -613/-603(CAE2) and -781/-770(CAE3) respectively were identified which may act as activator or repressor in either prestalk or prespore specific manner. To understand the significance of these elements and validate its predicted role, further deletion analysis was performed. A strong slugspecific prestalk repressor and a general activator were identified at -603/-569 (includes CAE1) and -804/-764 (includes CAE3) respectively suggesting CAEs are important for regulating pkcA expression as in many Dictyostelium promoters. This study suggests that pkcA promoter encompasses both prestalk and prespore activator elements but it is suppressed in prestalk cells during development. This suppression is brought by different regions of pkcA promoter at different developmental stages.