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Highlights • A straightforward method for optimising Trypanosoma cruzi transfection efficiency.• Facilitated by hydroxyurea-induced cell-cycle synchronization.• Applicable to both episomal and integrative-mediated transformation.• Reduces the time required to generate genetically… Click to show full abstract
Highlights • A straightforward method for optimising Trypanosoma cruzi transfection efficiency.• Facilitated by hydroxyurea-induced cell-cycle synchronization.• Applicable to both episomal and integrative-mediated transformation.• Reduces the time required to generate genetically modified cell lines.• Increases the number of stably transformed clones.
Keywords:
cell cycle;
hydroxyurea induced;
cell;
trypanosoma cruzi;
induced cell
Journal Title: Molecular and Biochemical Parasitology
Year Published: 2018
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Journal Title: Molecular and Biochemical Parasitology
Year Published: 2018
Link to full text (if available)
Share on Social Media: Sign Up to like & get
recommendations!